Ozone Prophylactic Effect and Antibiotics as a Modulator of Inflamatory Septic process in Rats

192 Wistar male rats (180200 g) were used to evaluate the prophylactic effect of ozone combined with antibiotics in septic shock model. The proinflammatory cytokines (IL-1β, TNFα, IL-6 and IL-2) expression were also determinated in different haemathopoyetic organs. The animals were taken at random and divided into 16 groups, 12 animals each: Group A, positive control (sepsis without treatment); groups B and C treated with ozone (200 ug / 250 g b. w) and Cefotaxim (25mg/kg), (0h, +1h) and (+1 h) respectivitly; groups D and E, ozone and Cefodizim (25mg/kg (0h, +1h) and (+1 h) respectively; groups F and G ozone and levofloxacin (12.5 mg/kg), (0h, +1h) and (+1 h) respectively; groups H and I ozone and Piperacillin /Tazobactam (65mg/kg), (0h, +1h) and (+1 h) respectively. The septic shock was carried out by single intraperitoneal injection of fecal material (0.65 g/kg b/w) from a donor rat, diluted in saline solution 0.9%. The other groups (J-P) of animals were treated only with antibiotics. The group pretreated with ozone and antibiotics showed a significant increase in the survival rate and showed also a decrease of IL-1β mRNA expression in liver. Ozone pretreatment might prevent lethal peritonitis by controlling inflammatory process generated physiologically into the body against specific damage or specific organism.